2024
An evolutionarily conserved ubiquitin ligase drives infection and transmission of flaviviruses
Wu L, Zhang L, Feng S, Chen L, Lin C, Wang G, Zhu Y, Wang P, Cheng G. An evolutionarily conserved ubiquitin ligase drives infection and transmission of flaviviruses. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2317978121. PMID: 38593069, PMCID: PMC11032495, DOI: 10.1073/pnas.2317978121.Peer-Reviewed Original ResearchConceptsNonstructural proteinsSingle-stranded RNA genomeER-associated degradationE3 ligase HRD1Individual functional proteinsFlavivirus infectionCellular fitnessRNA genomeUbiquitin systemFlavivirus NS4AFunctional proteinsFlavivirus proteinsLysine residuesMammalian hostsMosquito-borne flavivirusSmall molecule inhibitorsStructural proteinsHrd1Viral proteinsProgeny virionsUbiquitinPotential therapeutic targetDENV2 infectionProteinNS4A
2021
A pentameric protein ring with novel architecture is required for herpesviral packaging
Didychuk A, Gates S, Gardner M, Strong L, Martin A, Glaunsinger B. A pentameric protein ring with novel architecture is required for herpesviral packaging. ELife 2021, 10: e62261. PMID: 33554858, PMCID: PMC7889075, DOI: 10.7554/elife.62261.Peer-Reviewed Original ResearchConceptsViral genomeAccessory factorsBind double-stranded DNAPositively charged central channelContext of KSHV infectionDouble-stranded DNA virusesPositively charged residuesOncogenic herpesvirus Kaposi's sarcoma-associated herpesvirusKaposi's sarcoma-associated herpesvirusDouble-stranded DNASarcoma-associated herpesvirusGenome packagingHomologous proteinsNascent capsidsDNA bindingProtein ringGenomePackaging motorDNA virusesCharged residuesProgeny virionsMolecular motorsCentral channelKSHV infectionMutants
2016
Discovery of a Coregulatory Interaction between Kaposi's Sarcoma-Associated Herpesvirus ORF45 and the Viral Protein Kinase ORF36
Avey D, Tepper S, Pifer B, Bahga A, Williams H, Gillen J, Li W, Ogden S, Zhu F. Discovery of a Coregulatory Interaction between Kaposi's Sarcoma-Associated Herpesvirus ORF45 and the Viral Protein Kinase ORF36. Journal Of Virology 2016, 90: 5953-5964. PMID: 27099309, PMCID: PMC4907238, DOI: 10.1128/jvi.00516-16.Peer-Reviewed Original ResearchConceptsKaposi's sarcoma-associated herpesvirusKSHV ORF36Viral protein kinaseSarcoma-associated herpesvirusProtein kinaseKSHV lytic replicationKaposi's Sarcoma-Associated Herpesvirus ORF45Infectious progeny virionsHerpesvirus protein kinasesProgeny virionsLytic replicationKinase-dead mutantBacterial artificial chromosome mutagenesisBasic N-terminusCoregulatory interactionsChromosome mutagenesisORF45 proteinViral life cycleAcidic patchKSHV proteinsORF36Human tumor virusKinase activityORF45N-terminus
2015
ORF33 and ORF38 of Kaposi's Sarcoma-Associated Herpesvirus Interact and Are Required for Optimal Production of Infectious Progeny Viruses
Wu J, Avey D, Li W, Gillen J, Fu B, Miley W, Whitby D, Zhu F. ORF33 and ORF38 of Kaposi's Sarcoma-Associated Herpesvirus Interact and Are Required for Optimal Production of Infectious Progeny Viruses. Journal Of Virology 2015, 90: 1741-1756. PMID: 26637455, PMCID: PMC4734004, DOI: 10.1128/jvi.02738-15.Peer-Reviewed Original ResearchConceptsProgeny virionsCytoplasmic vesiclesKaposi's Sarcoma-Associated Herpesvirus InteractsDouble null mutantsEarly endosome membraneVirion-containing vesiclesMembranes of vesiclesLytic replicationViral gene expressionProgeny virion productionViral lytic replicationEndosome membraneHerpesvirus assemblyGolgi membranesComponent proteinsIdentical phenotypesGene expressionDelicate regulationVirion proteinsVirus assemblyVirus propagationExact functionInfectious progeny virusMature virionsViral assemblyA Survey of the Interactome of Kaposi's Sarcoma-Associated Herpesvirus ORF45 Revealed Its Binding to Viral ORF33 and Cellular USP7, Resulting in Stabilization of ORF33 That Is Required for Production of Progeny Viruses
Gillen J, Li W, Liang Q, Avey D, Wu J, Wu F, Myoung J, Zhu F. A Survey of the Interactome of Kaposi's Sarcoma-Associated Herpesvirus ORF45 Revealed Its Binding to Viral ORF33 and Cellular USP7, Resulting in Stabilization of ORF33 That Is Required for Production of Progeny Viruses. Journal Of Virology 2015, 89: 4918-4931. PMID: 25694600, PMCID: PMC4403494, DOI: 10.1128/jvi.02925-14.Peer-Reviewed Original ResearchConceptsUbiquitin-specific protease 7Kaposi's sarcoma-associated herpesvirusSarcoma-associated herpesvirusKSHV lytic replicationORF45 proteinKaposi's Sarcoma-Associated Herpesvirus ORF45Lytic replicationCarboxyl-terminal 19 amino acidsKSHV ORF45Copurified proteinsConsensus motifMultifunctional proteinKinase proteinInteractomeCarboxyl terminusKSHV genomeORF45Tegument proteinsWeight complexesProtein accumulationORF33Progeny virionsAmino acidsHuman cancersProtein
2013
Distinct host cell fates for human malignant melanoma targeted by oncolytic rodent parvoviruses
Vollmers EM, Tattersall P. Distinct host cell fates for human malignant melanoma targeted by oncolytic rodent parvoviruses. Virology 2013, 446: 37-48. PMID: 24074565, PMCID: PMC3811133, DOI: 10.1016/j.virol.2013.07.013.Peer-Reviewed Original ResearchConceptsPrimary human melanoma cellsPatient-derived melanoma cell linesCurrent therapeutic regimensHuman malignant melanomaRodent parvovirusesHuman melanoma cellsLow response rateMelanoma cell linesOncolytic parvovirusTherapeutic regimensMetastatic melanomaMalignant melanomaResponse rateVirion uptakeViral protein synthesisHost cell fateChimeric virusesMelanoma cellsComplete lysisCell linesMelanomaViral transcriptionParvovirusProgeny virionsLow multiplicity
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