2024
Intestinal Nogo-B reduces GLP1 levels by binding to proglucagon on the endoplasmic reticulum to inhibit PCSK1 cleavage
Gong K, Xue C, Feng Z, Pan R, Wang M, Chen S, Chen Y, Guan Y, Dai L, Zhang S, Jiang L, Li L, Wang B, Yin Z, Ma L, Iwakiri Y, Tang J, Liao C, Chen H, Duan Y. Intestinal Nogo-B reduces GLP1 levels by binding to proglucagon on the endoplasmic reticulum to inhibit PCSK1 cleavage. Nature Communications 2024, 15: 6845. PMID: 39122737, PMCID: PMC11315690, DOI: 10.1038/s41467-024-51352-3.Peer-Reviewed Original ResearchConceptsEnteroendocrine cellsEndoplasmic reticulum (ER)-resident proteinGlucagon-like peptide 1Nogo-BEndoplasmic reticulumStimulate insulin secretionPotential therapeutic targetProglucagonGlucagon-like peptide 1 receptorInhibit glucagon secretionRegulatory processesIntestinal tractProglucagon fragmentInsulin secretionCleavageNogo-B knockoutTherapeutic targetPancreatic cellsPeptide 1Glucagon secretionCellsReticulonGolgiReticulon 4BInsulin resistance
2006
Identifying glucagon-like peptide-1 mimetics using a novel functional reporter gene high-throughput screening assay
Chen J, Bai G, Yang Y, Geng P, Cao Y, Zhu Y. Identifying glucagon-like peptide-1 mimetics using a novel functional reporter gene high-throughput screening assay. Peptides 2006, 28: 928-934. PMID: 17267075, DOI: 10.1016/j.peptides.2006.12.012.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBlood GlucoseCHO CellsCricetinaeCricetulusDose-Response Relationship, DrugEnzyme-Linked Immunosorbent AssayFemaleGene Expression RegulationGlucagon-Like Peptide 1Glucagon-Like Peptide-1 ReceptorGlucose Tolerance TestGreen Fluorescent ProteinsHypoglycemic AgentsInjections, IntraperitonealMicePeptide LibraryPeptidesRatsReceptors, GlucagonTransfectionConceptsGlucagon-like peptide-1High-throughput screeningRounds of selectionChinese Kunming miceEGFP reporter geneHigh-throughput screening assayGreen fluorescent proteinDodecapeptide libraryPositional cloningResistant to DPP-IVRecombinant cell linesKS-12Reporter geneGlucagon-like peptide-1 mimeticsFluorescent proteinPeptide libraryGlucagon-like peptide-1 receptorGlucagon-like peptide-1 analogInhibit glucagon secretionMonitor receptor activityCell linesDPP-IVDipeptidyl peptidase IVDose-dependent mannerScreening assay
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