2025
Tracing the origins of fibrotic fibroblasts: does the name matter? Look at the genes!
Mailleux A, Justet A. Tracing the origins of fibrotic fibroblasts: does the name matter? Look at the genes! European Respiratory Journal 2025, 65: 2402170. PMID: 39915043, DOI: 10.1183/13993003.02170-2024.Peer-Reviewed Original Research
2024
Rare genetic variation in fibronectin 1 (FN1) protects against APOE ɛ4 in Alzheimer’s Disease
Bhattarai P, Gunasekaran T, Uzrek B, Reyes‐Dumeyer D, Jülich D, Lee A, Yilmaz E, Tayran H, Lantigua R, Medrano M, Mejia D, Recio P, Flaherty D, Dalgard C, Nuriel T, Ertekin‐Taner N, Dickson D, Teich A, Holley S, Mayeux R, Kizil C, Vardarajan B. Rare genetic variation in fibronectin 1 (FN1) protects against APOE ɛ4 in Alzheimer’s Disease. Alzheimer's & Dementia 2024, 20: e089111. PMCID: PMC11710415, DOI: 10.1002/alz.089111.Peer-Reviewed Original ResearchWhole-genome sequencingLoss-of-functionIn vivo functional studiesFibronectin 1Genetic variationAlzheimer's diseaseFunctional studiesWhole-genome sequence analysisTarget genesRare genetic variationLoss-of-function mutationsPotential gene variantsZebrafish modelGenome sequenceProtective variantsAPOE variantsGenetic variantsECM proteinsZebrafish AD modelBioinformatics analysisAD pathologyPotential therapeutic interventional targetsPathway analysisPostmortem human brain tissueRare variantsTANGO1 inhibitors reduce collagen secretion and limit tissue scarring
Raote I, Rosendahl A, Häkkinen H, Vibe C, Küçükaylak I, Sawant M, Keufgens L, Frommelt P, Halwas K, Broadbent K, Cunquero M, Castro G, Villemeur M, Nüchel J, Bornikoel A, Dam B, Zirmire R, Kiran R, Carolis C, Andilla J, Loza-Alvarez P, Ruprecht V, Jamora C, Campelo F, Krüger M, Hammerschmidt M, Eckes B, Neundorf I, Krieg T, Malhotra V. TANGO1 inhibitors reduce collagen secretion and limit tissue scarring. Nature Communications 2024, 15: 3302. PMID: 38658535, PMCID: PMC11043333, DOI: 10.1038/s41467-024-47004-1.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulum exit sitesECM proteinsSecretion of ECM proteinsPeptide inhibitorFibrotic diseasesCollagen exportTANGO1Binding interfaceWound healingZebrafish resultsECM componentsReduced granulation tissue formationGranulation tissue formationEffective therapyCutaneous wound healingInhibitor treatmentFibrotic processUncontrolled secretionWidespread occurrenceProtein levelsExit siteExcessive scarringAmount of collagenTherapeutic modulationTissue scarringRare genetic variation in fibronectin 1 (FN1) protects against APOEε4 in Alzheimer’s disease
Bhattarai P, Gunasekaran T, Belloy M, Reyes-Dumeyer D, Jülich D, Tayran H, Yilmaz E, Flaherty D, Turgutalp B, Sukumar G, Alba C, McGrath E, Hupalo D, Bacikova D, Le Guen Y, Lantigua R, Medrano M, Rivera D, Recio P, Nuriel T, Ertekin-Taner N, Teich A, Dickson D, Holley S, Greicius M, Dalgard C, Zody M, Mayeux R, Kizil C, Vardarajan B. Rare genetic variation in fibronectin 1 (FN1) protects against APOEε4 in Alzheimer’s disease. Acta Neuropathologica 2024, 147: 70. PMID: 38598053, PMCID: PMC11006751, DOI: 10.1007/s00401-024-02721-1.Peer-Reviewed Original ResearchConceptsLoss-of-functionWhole-genome sequencingFibronectin 1Genetic variationAlzheimer's diseaseAD riskRare coding variantsLoss-of-function variantsRare genetic variationGene Ontology termsFamily based studyIn vivo functional studiesAD-related pathologyAlpha 2 chainOntology termsPresence of cellular mechanismsProtective variantsECM proteinsAD pathologyPathway analysisFunctional studiesUnaffected carriersZebrafish modelAPOEe4 alleleProtein levels
2021
Global proteomic analysis of extracellular matrix in mouse and human brain highlights relevance to cerebrovascular disease
Pokhilko A, Brezzo G, Handunnetthi L, Heilig R, Lennon R, Smith C, Allan S, Granata A, Sinha S, Wang T, Markus H, Naba A, Fischer R, Van Agtmael T, Horsburgh K, Cader M. Global proteomic analysis of extracellular matrix in mouse and human brain highlights relevance to cerebrovascular disease. Cerebrovascular And Brain Metabolism Reviews 2021, 41: 2423-2438. PMID: 33730931, PMCID: PMC8392779, DOI: 10.1177/0271678x211004307.Peer-Reviewed Original Research
2019
Extracellular matrix-derived biomaterials in engineering cell function
Xing H, Lee H, Luo L, Kyriakides TR. Extracellular matrix-derived biomaterials in engineering cell function. Biotechnology Advances 2019, 42: 107421. PMID: 31381963, PMCID: PMC6995418, DOI: 10.1016/j.biotechadv.2019.107421.Peer-Reviewed Educational MaterialsConceptsTissue-derived extracellular matrixECM proteinsExtracellular matrixCell-derived extracellular matrixSingle ECM proteinsStem cell differentiationCell functionCell-specific responsesGuide cell migrationEngineering of biomaterialsCell differentiationECM environmentECM polymersCell adhesionNative stateCell migrationProteinUse of biomaterialsMaterial coatingEnhanced cell adhesionBiomaterialsCell isolationDecellularization technologyRecent advancesDermal repairThrombospondin-2 regulates extracellular matrix production, LOX levels, and cross-linking via downregulation of miR-29
Calabro NE, Barrett A, Chamorro-Jorganes A, Tam S, Kristofik NJ, Xing H, Loye AM, Sessa WC, Hansen K, Kyriakides TR. Thrombospondin-2 regulates extracellular matrix production, LOX levels, and cross-linking via downregulation of miR-29. Matrix Biology 2019, 82: 71-85. PMID: 30876926, PMCID: PMC6710120, DOI: 10.1016/j.matbio.2019.03.002.Peer-Reviewed Original ResearchConceptsECM protein productionProtein productionLysyl oxidaseThrombospondin-2MiR-29Matricellular proteinQuantitative proteomics approachLevels of LOXExtracellular matrix productionProteomic approachECM proteinsMajor regulatorECM homeostasisExtracellular matrixMatrix productionProteinCollagen fibrillogenesisMiR-29 expressionFibrillar collagenImportant modulatorDependent processesLOX levelsRegulatorProductionHomeostasis
2016
Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices
Calle EA, Hill RC, Leiby KL, Le AV, Gard AL, Madri JA, Hansen KC, Niklason LE. Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices. Acta Biomaterialia 2016, 46: 91-100. PMID: 27693690, PMCID: PMC5451113, DOI: 10.1016/j.actbio.2016.09.043.Peer-Reviewed Original ResearchConceptsExtracellular matrixLung extracellular matrixMatrix proteinsECM-associated proteinsCell-matrix interactionsProtein extraction methodsWhole organ regenerationRegenerative medicineOrganotypic cell culturesQuantitative proteomicsAcellular extracellular matrixECM proteinsOrgan regenerationCell adhesionProteomicsProtein analysisQuantitative biochemical dataProteinPotent substrateXenogeneic extracellular matrixTargeted proteomicsCell nuclear antigenBiochemical dataImportant glycoproteinCell cultures
2015
Proteomic Analysis of the Pericyte Derived Extracellular Matrix
Brown L, Sava P, Garcia C, Gonzalez A. Proteomic Analysis of the Pericyte Derived Extracellular Matrix. Cellular And Molecular Bioengineering 2015, 8: 349-363. DOI: 10.1007/s12195-015-0408-5.Peer-Reviewed Original ResearchMatrix proteinsStructural ECM proteinsBasic cellular responsesExtracellular matrix proteinsFunctional annotationProteomic analysisRegulatory signalsCell rufflingECM proteinsCellular responsesWestern blot analysisECM componentsExtracellular matrixEndothelial cell tubule formationFirst characterizationProteinMigratory velocityBlot analysisTubule formationECMNeutrophil polarizationStructural collagenVascular integrityLC/MS-MSBasement membrane
2014
Reversible Modulation of Myofibroblast Differentiation in Adipose-Derived Mesenchymal Stem Cells
Desai VD, Hsia HC, Schwarzbauer JE. Reversible Modulation of Myofibroblast Differentiation in Adipose-Derived Mesenchymal Stem Cells. PLOS ONE 2014, 9: e86865. PMID: 24466271, PMCID: PMC3900664, DOI: 10.1371/journal.pone.0086865.Peer-Reviewed Original ResearchMeSH KeywordsAdipose TissueBlotting, WesternCell DifferentiationCell MovementCells, CulturedExtracellular Matrix ProteinsFibroblast Growth Factor 2HumansMesenchymal Stem CellsMyofibroblastsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSignal TransductionConceptsAdipose-derived mesenchymal stem cellsMesenchymal stem cellsExtracellular matrixERK/MAP kinaseMyofibroblastic phenotypeStem cellsRegulation of tenascinProtein type IActivation of Smad2Abundant extracellular matrixFocal adhesionsGrowth factorKinase downstreamHuman adipose-derived mesenchymal stem cellsCytoskeletal proteinsMAP kinaseStress fibersECM proteinsCell differentiationADSC differentiationDifferentiation processFibroblast-like cellsMyofibroblast differentiationNovel therapeutic strategiesRegenerative medicine
2012
Approaches in extracellular matrix engineering for determination of adhesion molecule mediated single cell function
Ayres-Sander C, Gonzalez A. Approaches in extracellular matrix engineering for determination of adhesion molecule mediated single cell function. Frontiers In Biology 2012, 8: 32-49. DOI: 10.1007/s11515-012-1199-x.Peer-Reviewed Original ResearchCell-ECM interactionsCell adhesion moleculeExtracellular matrixThree-dimensional matrixCellular processesNative extracellular matrixAdhesion moleculesTwo-dimensional substratesSingle cell functionCell functionCell movementECM proteinsNative proteinPeptide sequencesIntegrin expressionBioactive peptide sequencesCell functionalityMatrix engineeringProteinHuman tissuesCellsDiseased tissuesCadherinMoleculesTissue
2003
Glucocorticoid Enhances Transforming Growth Factor-β Effects on Extracellular Matrix Protein Expression in Human Placental Mesenchymal Cells1
Lee MJ, Ma Y, LaChapelle L, Kadner SS, Guller S. Glucocorticoid Enhances Transforming Growth Factor-β Effects on Extracellular Matrix Protein Expression in Human Placental Mesenchymal Cells1. Biology Of Reproduction 2003, 70: 1246-1252. PMID: 14681196, DOI: 10.1095/biolreprod.103.021956.Peer-Reviewed Original ResearchConceptsPlacental mesenchymal cellsIntrauterine growth restrictionFFN levelsProtein expressionCol IV expressionInteraction of glucocorticoidsDose-dependent mannerHuman term placentaFibronectin protein levelsExtracellular matrix protein expressionUncomplicated pregnanciesPolymerase chain reaction analysisReal-time polymerase chain reaction analysisGrowth restrictionDEX treatmentChain reaction analysisECM proteinsPlacental villiTerm placentaECM protein expressionCol IIIPlacental architectureMatrix protein expressionDexamethasoneGlucocorticoids
2000
Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis
Willis A, Fuse S, Wang X, Chen E, Tuszynski G, Sumpio B, Gahtan V. Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis. Journal Of Vascular Surgery 2000, 31: 1160-1167. PMID: 10842153, DOI: 10.1067/mva.2000.106489.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell CountCell DivisionCells, CulturedChemotaxisChromonesEnzyme InhibitorsExtracellular Matrix ProteinsFibronectinsHyperplasiaIndolesMaleimidesMorpholinesMuscle, Smooth, VascularPhosphoinositide-3 Kinase InhibitorsProtein Kinase CSignal TransductionStatistics as TopicThrombospondin 1Tunica IntimaVitronectinConceptsProtein kinase CVSMC migrationLY-294002Thrombospondin-1ECM proteinsKinase CSmooth muscle cell chemotaxisSerum-free mediumCellular signaling pathwaysInhibition of phosphatidylinositolVascular smooth muscle cell chemotaxisVascular smooth muscle cell migrationPI3K inhibitorsSmooth muscle cell migrationSignaling pathwaysMuscle cell migrationBisindolylmaleimide ICell migrationPKC inhibitorGF 109203XExtracellular matrix depositionTop wellsK inhibitorsAortic VSMCsCell chemotaxis
1998
Distinct signal transduction pathways are utilized during the tube formation and survival phases of in vitro angiogenesis
Ilan N, Mahooti S, Madri J. Distinct signal transduction pathways are utilized during the tube formation and survival phases of in vitro angiogenesis. Journal Of Cell Science 1998, 111: 3621-3631. PMID: 9819353, DOI: 10.1242/jcs.111.24.3621.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCalcium-Calmodulin-Dependent Protein KinasesCapillariesCell Culture TechniquesCell LineCell SurvivalCollagenEndothelial Growth FactorsEndothelium, VascularExtracellular MatrixHumansLymphokinesNeovascularization, PhysiologicPhosphatidylinositol 3-KinasesPhosphorylationProtein Kinase CProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktSignal TransductionTetradecanoylphorbol AcetateVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsHuman umbilical vein endothelial cellsAkt/PKB pathwayTube formationDistinct signal transduction pathwaysAkt/PKBSignal transduction pathwaysDifferent ECM proteinsCollagen gelsExtracellular matrix componentsPeptide growth factorsPKB pathwayProtein kinaseTransduction pathwaysMAP kinaseUmbilical vein endothelial cellsECM proteinsVein endothelial cellsNew blood vesselsPre-existing onesKinaseMajor groupsVivo angiogenesisRapid inductionMatrix componentsSurvival phase
1986
Mechanisms of cytoskeletal regulation: Modulation of aortic endothelial cell protein band 4.1 by the extracellular matrix
Leto T, Pratt B, Madri J. Mechanisms of cytoskeletal regulation: Modulation of aortic endothelial cell protein band 4.1 by the extracellular matrix. Journal Of Cellular Physiology 1986, 127: 423-431. PMID: 3519624, DOI: 10.1002/jcp.1041270311.Peer-Reviewed Original ResearchConceptsActin filamentsExtracellular matrixProtein 4.1Band 4.1Contact inhibitionCytoplasmic actin filamentsTransduction of informationCell-cell contactCortical membrane proteinsErythroid proteinCytoskeletal regulationSpecific cleavage productsMembrane proteinsSkeletal proteinsCysteine residuesCell cytoskeletonEndothelial cellsFilamentous distributionECM proteinsEndothelial cell cytoskeletonFibronectin substrateReorganization eventsCell nucleiComplex pathwaysImmunoblot analysis
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