2025
Effect of endometriosis-linked microRNAs on hepatic gene expression
Mamillapalli R, Slutzky R, Mangla A, Gawde N, Taylor H. Effect of endometriosis-linked microRNAs on hepatic gene expression. F&S Science 2025 PMID: 39971156, DOI: 10.1016/j.xfss.2025.02.001.Peer-Reviewed Original ResearchMiR-3613-5pMiR-let-7bMiR-125b-5pMiR-150MiR-150-5pCirculating microRNAsMicroRNAsReactionExpression of IGFBP1Quantitative polymerase chain reactionSuppress target genesExpression of MRC1Polymerase chain reactionBinding sitesReal-time quantitative polymerase chain reactionHepatic gene expressionExpression of CYP2R1Bioinformatics analysisEffect of endometriosisSerum of womenCirculation of womenGene expressionChain reactionMiRsHepatic cells
2024
Prenatal arsenic metabolite exposure is associated with increased newborn mitochondrial DNA copy number: evidence from a birth cohort study
Qiu F, Zhang H, Wang X, Jia Z, He Y, Wu Y, Li Z, Zheng T, Xia W, Xu S, Li Y. Prenatal arsenic metabolite exposure is associated with increased newborn mitochondrial DNA copy number: evidence from a birth cohort study. Environmental Science And Pollution Research 2024, 31: 38142-38152. PMID: 38789711, DOI: 10.1007/s11356-024-32933-7.Peer-Reviewed Original ResearchDNA copy numberThird trimesterUrinary arsenic species concentrationsNewborn mitochondrial DNA copy numberMother-newborn pairsDose-response trendMitochondria DNA copy numberReal-time polymerase chain reactionBirth cohort studyMetabolite exposureCopy numberMitochondrial DNA copy numberOne-unit incrementCovariate-adjusted modelsBlood mtDNAcnQuantitative real-time polymerase chain reactionPolymerase chain reactionCohort studyTrimesterMetabolite levelsPotential associationStatistical significanceArsenic metabolitesChain reactionMtDNAcnCoupling clearing and hybridization chain reaction approaches to investigate gene expression in organs inside intact insect heads
Cayrol B, Colella S, Uzest M. Coupling clearing and hybridization chain reaction approaches to investigate gene expression in organs inside intact insect heads. Microscopy Research And Technique 2024, 87: 1926-1932. PMID: 38558482, DOI: 10.1002/jemt.24561.Peer-Reviewed Original ResearchConceptsSecretory cellsSpatial distribution of transcriptsSecretory cells of salivary glandsDetected RNA moleculesDetection of transcriptsDistribution of transcriptsInvestigate gene expressionPigmented cuticleHigh-resolution analysisRNA moleculesCells of salivary glandsHybridization chain reactionAphid headsGene expressionPrincipal glandChain reactionTranscriptionAmplification approachInsect headAcyrthosiphon pisumHybridisation chain reactionCuticleMultiplex approachSmall organismsSHP
2023
Rhinoviruses
Landry M, Lu X. Rhinoviruses. 2023, 1-15. DOI: 10.1002/9781683674849.mcm0092.Peer-Reviewed Original ResearchRV isolatesDevelopment of clinical symptomsLower respiratory tract diseaseMediators of inflammationChemical mediators of inflammationReverse transcription polymerase chain reactionTranscription polymerase chain reactionRV infectionType-specific antiseraClinical symptomsPolymerase chain reactionPresumptive diagnosisRespiratory tract diseaseMolecular typingGold standardIncreased susceptibilityPredominant siteCell linesCytopathic effectChain reactionInadequate sleepViral diagnosticsCell culturesSerotypesSymptomsRhinoviruses
Landry M, Lu X. Rhinoviruses. 2023, 1-15. DOI: 10.1002/9781683670438.mcm0092.Peer-Reviewed Original ResearchRV isolatesDevelopment of clinical symptomsLower respiratory tract diseaseMediators of inflammationChemical mediators of inflammationReverse transcription polymerase chain reactionTranscription polymerase chain reactionRV infectionType-specific antiseraClinical symptomsPolymerase chain reactionPresumptive diagnosisRespiratory tract diseaseMolecular typingGold standardIncreased susceptibilityPredominant siteCell linesCytopathic effectChain reactionInadequate sleepViral diagnosticsCell culturesSerotypesSymptomsRole of fungal burden in risk stratification of HIV-negative patients with Pneumocystis pneumonia: A 12-year, retrospective, observational, multicenter cohort
Grønseth S, Rogne T, Heggelund L, Åsvold B, Afset J, Damås J. Role of fungal burden in risk stratification of HIV-negative patients with Pneumocystis pneumonia: A 12-year, retrospective, observational, multicenter cohort. International Journal Of Infectious Diseases 2023, 134: 177-186. PMID: 37339716, DOI: 10.1016/j.ijid.2023.06.013.Peer-Reviewed Original ResearchConceptsCharlson Comorbidity IndexHIV-negative patientsPneumocystis pneumoniaRisk stratificationFungal burdenMulticenter cohortPolymerase chain reactionAbnormal leukocyte countComorbid cardiovascular diseaseLow serum albuminBronchoalveolar lavage fluidC-reactive proteinChain reactionHigher fungal burdenReal-time polymerase chain reactionProbable Pneumocystis pneumoniaCycle threshold valuesComorbidity indexCorticosteroid useLavage fluidSemiquantitative real-time polymerase chain reactionLeukocyte countImmunological disordersCardiovascular diseaseRetrospective analysisCharacteristics and Treatments of Coronavirus Disease 2019 Patients Presenting With Persistent Hiccups: A Scoping Review
Boulis M, Boulis M, Cosgrove M, He K. Characteristics and Treatments of Coronavirus Disease 2019 Patients Presenting With Persistent Hiccups: A Scoping Review. Clinical Neuropharmacology 2023, 46: 157-163. PMID: 37212668, DOI: 10.1097/wnf.0000000000000554.Peer-Reviewed Original ResearchPersistent hiccupsCOVID-19 patientsChest imagingCharacteristics of COVID-19 patientsReverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionManifestations of COVID-19Reverse transcriptase-polymerase chain reaction testSymptoms of infectionAtypical presentationLung involvementFavorable outcomeTreatment optionsDifferential diagnosisTreatment of coronavirus diseasePatientsCoronavirus disease 2019HiccupsChain reactionMetoclopramideReaction testCOVID-19Scoping reviewTreatmentChlorpromazine
2022
Laboratory Diagnosis for SARS-CoV-2 Infection
Christensen BB, Azar MM, Turbett SE. Laboratory Diagnosis for SARS-CoV-2 Infection. Infectious Disease Clinics Of North America 2022, 36: 327-347. PMID: 35636903, PMCID: PMC9141924, DOI: 10.1016/j.idc.2022.02.002.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionClinical syndromeMolecular testingPatient's clinical syndromeReverse transcriptase-polymerase chain reactionOptimal diagnostic testCOVID-19 infectionSymptom onsetAcute infectionAntigen testingSerologic testingAcute diagnosisPolymerase chain reactionLaboratory diagnosisDiagnostic testsInfectionChain reactionDiagnosisSyndromeTestingShorter Telomeres Related to Posttraumatic Stress Disorder Re-experiencing Symptoms in Sexually Assaulted Civilian Women
Carvalho CM, Coimbra BM, Xavier G, Bugiga AVG, Fonseca T, Olff M, Polimanti R, Mello AF, Ota VK, Mello MF, Belangero SI. Shorter Telomeres Related to Posttraumatic Stress Disorder Re-experiencing Symptoms in Sexually Assaulted Civilian Women. Frontiers In Psychiatry 2022, 13: 835783. PMID: 35664481, PMCID: PMC9161278, DOI: 10.3389/fpsyt.2022.835783.Peer-Reviewed Original ResearchPosttraumatic stress disorderRe-experiencing symptomsPolymerase chain reactionMini International Neuropsychiatric InterviewClinician-Administered PTSD ScaleInternational Neuropsychiatric InterviewPTSD re-experiencing symptomsReal-time polymerase chain reactionCivilian womenMultiplex real-time polymerase chain reactionRemitter patientsShorter leukocyteBaseline LTLNeuropsychiatric InterviewPrevious historyPTSD ScaleMental disordersPTSD diagnosisSymptomsStress disorderPTSD casesPTSD symptomsTime pointsWomenChain reactionPredominance of Severe Plasma Leakage in Pediatric Patients With Severe Dengue in Puerto Rico
Paz-Bailey G, Sánchez-González L, Torres-Velasquez B, Jones E, Perez-Padilla J, Sharp T, Lorenzi O, Delorey M, Munoz-Jordan J, Tomashek K, Waterman S, Alvarado L, Rivera-Amill V. Predominance of Severe Plasma Leakage in Pediatric Patients With Severe Dengue in Puerto Rico. The Journal Of Infectious Diseases 2022, 226: 1949-1958. PMID: 35510941, PMCID: PMC10015274, DOI: 10.1093/infdis/jiac165.Peer-Reviewed Original ResearchConceptsSevere plasma leakageSevere dengueReverse transcriptase-polymerase chain reactionPlasma leakageSevere bleedingDengue virusHistory of feverDays of presentationTranscriptase-polymerase chain reactionYear oldsNonsevere dengueOrgan involvementCommon presentationPediatric patientsClinical manifestationsDengue patientsEmergency departmentLaboratory findingsIgM enzymePatientsSerum samplesImmunosorbent assayDengueChain reactionBleedingSaliVISION: a rapid saliva-based COVID-19 screening and diagnostic test with high sensitivity and specificity
DeFina SM, Wang J, Yang L, Zhou H, Adams J, Cushing W, Tuohy B, Hui P, Liu C, Pham K. SaliVISION: a rapid saliva-based COVID-19 screening and diagnostic test with high sensitivity and specificity. Scientific Reports 2022, 12: 5729. PMID: 35388102, PMCID: PMC8986854, DOI: 10.1038/s41598-022-09718-4.Peer-Reviewed Original ResearchConceptsSARS-CoV-2Severe acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2Diagnostic testingRespiratory syndrome coronavirus 2Reverse transcription loop-mediated isothermal amplificationTranscription loop-mediated isothermal amplificationTranscription-quantitative polymerase chain reactionSyndrome coronavirus 2COVID-19 diagnostic testingCoronavirus disease 2019Public health challengeRapid diagnostic testingCOVID-19 screeningQuantitative polymerase chain reactionLoop-mediated isothermal amplificationCoronavirus 2Polymerase chain reactionLaboratory testingRapid diagnostic assaysDisease 2019RT-LAMPInvasive sample collectionDiagnostic testsChain reactionRelative contribution of low-density and asymptomatic infections to Plasmodium vivax transmission in the Amazon: pooled analysis of individual participant data from population-based cross-sectional surveys
Ferreira MU, Corder RM, Johansen IC, Kattenberg JH, Moreno M, Rosas-Aguirre A, Ladeia-Andrade S, Conn JE, Llanos-Cuentas A, Gamboa D, Rosanas-Urgell A, Vinetz JM. Relative contribution of low-density and asymptomatic infections to Plasmodium vivax transmission in the Amazon: pooled analysis of individual participant data from population-based cross-sectional surveys. The Lancet Regional Health - Americas 2022, 9: 100169. PMID: 35663000, PMCID: PMC9161731, DOI: 10.1016/j.lana.2021.100169.Peer-Reviewed Original ResearchIndividual participant dataPolymerase chain reactionClinical manifestationsParasite densityMosquito infectionMalaria transmissionPopulation-based cross-sectional surveyParticipant dataMalaria elimination strategiesPopulation-based surveyHigh parasite densityCross-sectional surveyInfected childrenClinical symptomsAsymptomatic infectionUS National InstitutesAsymptomatic carriersParasite carriersInfectious reservoirVivax transmissionInfectionFundação de Amparo à PesquisaChain reactionNational InstituteElimination strategy
2021
Molecular evidence of Anaplasma phagocytophilum in olive baboons and vervet monkeys in Kenya
Masika SJ, Muchemi GM, Okumu TA, Mutura S, Zimmerman D, Kamau J. Molecular evidence of Anaplasma phagocytophilum in olive baboons and vervet monkeys in Kenya. BMC Veterinary Research 2021, 17: 385. PMID: 34906141, PMCID: PMC8669034, DOI: 10.1186/s12917-021-03095-2.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionAnaplasma phagocytophilumConventional polymerase chain reactionA. phagocytophilumTick-borne infectionsVervet monkeysPublic health implicationsZoonotic A. phagocytophilumOlive baboonsOverall prevalenceBlood samplesConclusionThis studyTropical diseasesGranulocytic anaplasmosisAnaplasma speciesChain reactionHealth implicationsIntracellular bacteriaBaboonsPhagocytophilumMonkeysPrevalenceObligate intracellular bacteriaIxodid ticksInfectionIncreased Detection of Mycobacterium tuberculosis Disease Using a Tissue-Based Laboratory-Developed Polymerase Chain Reaction Assay Compared to Standard Diagnostics.
Mackow NA, Abi-Raad R, Kerantzas CA, Hui P, Malinis M, Azar MM. Increased Detection of Mycobacterium tuberculosis Disease Using a Tissue-Based Laboratory-Developed Polymerase Chain Reaction Assay Compared to Standard Diagnostics. American Journal Of Tropical Medicine And Hygiene 2021, 105: 1657-1661. PMID: 34544041, PMCID: PMC8641361, DOI: 10.4269/ajtmh.21-0104.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedCulture TechniquesFemaleHumansLungLymph NodesMaleMiddle AgedMycobacterium tuberculosisPleuraReal-Time Polymerase Chain ReactionReference StandardsRetrospective StudiesSensitivity and SpecificitySputumTuberculosisTuberculosis, Lymph NodeTuberculosis, Multidrug-ResistantTuberculosis, PleuralTuberculosis, PulmonaryConceptsComposite reference standardMTB PCRAFB cultureMycobacterium tuberculosisPolymerase chain reactionAcid-fast bacilli smearMycobacterium tuberculosis diseasePositive AFB cultureChain reactionReal-time polymerase chain reactionStandard diagnosticsBacilli smearMTB casesTuberculosis diseaseClinical sensitivityLong turnaround timeXpertClinical performanceReference standardPCRVariable sensitivityTurnaround timeLymphPatientsTuberculosisBabesia microti: Pathogen Genomics, Genetic Variability, Immunodominant Antigens, and Pathogenesis
Puri A, Bajpai S, Meredith S, Aravind L, Krause PJ, Kumar S. Babesia microti: Pathogen Genomics, Genetic Variability, Immunodominant Antigens, and Pathogenesis. Frontiers In Microbiology 2021, 12: 697669. PMID: 34539601, PMCID: PMC8446681, DOI: 10.3389/fmicb.2021.697669.Peer-Reviewed Original ResearchPolymerase chain reactionPerinatal transmissionBlood transfusionModerate diseaseMost infectionsOrgan transplantationImmune evasionBlood smearsImmunodominant antigensHuman babesiosisInfection severityPathogenesisChain reactionPotential roleCell surface proteinsDiseaseDiagnosisHuman diseasesPathogen genomicsBabesiosisIxodid ticksPhylum ApicomplexaTransfusionTransplantationHumansInfant Nasopharyngeal Microbiota Subphenotypes and Early Childhood Lung Function: Evidence from a Rural Ghanaian Pregnancy Cohort
Dubowski K, Kaali S, Jack D, Prah RKD, Clemente JC, Tawiah T, Mujtaba M, Iddrisu L, Carrión D, Konadu DG, Agyei O, Kornu FM, Osei-Owusu S, Lee AG, Asante KP. Infant Nasopharyngeal Microbiota Subphenotypes and Early Childhood Lung Function: Evidence from a Rural Ghanaian Pregnancy Cohort. International Journal Of Environmental Research And Public Health 2021, 18: 7276. PMID: 34299726, PMCID: PMC8305530, DOI: 10.3390/ijerph18147276.Peer-Reviewed Original ResearchConceptsLung functionNasopharyngeal microbiotaPregnancy cohortLung healthPolymerase chain reactionRespiratory microbiotaLatent class analysisMassTag polymerase chain reactionGhana Randomized Air PollutionFuture lung healthChildhood lung functionSmall airway resistanceMultivariable linear regression modelsProspective pregnancy cohortAge four yearsAsymptomatic childrenMiddle-income countriesPulmonary diseaseAirway resistanceImpulse oscillometryNasopharyngeal swabsSubphenotypesCohortMore pathogensChain reactionIntegrating Parasitological and Entomological Observations to Understand Malaria Transmission in Riverine Villages in the Peruvian Amazon
Rosas-Aguirre A, Moreno M, Moreno-Gutierrez D, Llanos-Cuentas A, Saavedra M, Contreras-Mancilla J, Barboza J, Alava F, Aguirre K, Carrasco G, Prussing C, Vinetz J, Conn JE, Speybroeck N, Gamboa D. Integrating Parasitological and Entomological Observations to Understand Malaria Transmission in Riverine Villages in the Peruvian Amazon. The Journal Of Infectious Diseases 2021, 223: s99-s110. PMID: 33906225, PMCID: PMC8079135, DOI: 10.1093/infdis/jiaa496.Peer-Reviewed Original ResearchConceptsMalaria prevalenceMalaria transmissionRiverine villagesMalaria risk factorsMalaria transmission dynamicsSubmicroscopic infectionsLocal epidemiologyPolymerase chain reactionIncidence rateRisk factorsHigh prevalenceMalaria casesTreat interventionMalaria strategyPlasmodium vivaxPrevalenceChain reactionTransmission dynamicsAdult AnophelesHigh heterogeneityUrgent care center wait times increase for COVID-19 results in August 2020, with rapid testing availability limited
Yousman LC, Khunte A, Hsiang W, Jain S, Forman H, Wiznia D. Urgent care center wait times increase for COVID-19 results in August 2020, with rapid testing availability limited. BMC Health Services Research 2021, 21: 318. PMID: 33832506, PMCID: PMC8027963, DOI: 10.1186/s12913-021-06338-y.Peer-Reviewed Original ResearchConceptsUrgent care centersCOVID-19 testingTesting availabilityPolymerase chain reactionCare centerRapid testingPCR test resultsCosts of visitsChi-square testCOVID-19 testCOVID-19 resultsCommon locationSignificant decreaseStandardized scriptChain reactionCare ActCenter classificationT-testOverall lackResultsOur resultsVisitsHigher proportionCritical roleTestingMajorityTargeted RNAseq assay incorporating unique molecular identifiers for improved quantification of gene expression signatures and transcribed mutation fraction in fixed tumor samples
Fu C, Marczyk M, Samuels M, Trevarton AJ, Qu J, Lau R, Du L, Pappas T, Sinn BV, Gould RE, Pusztai L, Hatzis C, Symmans WF. Targeted RNAseq assay incorporating unique molecular identifiers for improved quantification of gene expression signatures and transcribed mutation fraction in fixed tumor samples. BMC Cancer 2021, 21: 114. PMID: 33541297, PMCID: PMC7860187, DOI: 10.1186/s12885-021-07814-8.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionParaffin-embedded tumor tissue samplesConcordance correlation coefficientFresh frozenFFPE samplesPrimary breast cancerMulti-gene signatureTumor tissue samplesActivating point mutationMutant allele fractionReverse transcriptionKey breast cancer genesGene expression signaturesBreast cancer genesPIK3CA mutationsBackgroundOur objectiveBreast cancerWhole transcriptome RNAseqTumor samplesLin's concordance correlation coefficientHormone receptorsFF samplesTissue samplesExpression signaturesChain reactionDepression, Religiosity, and Telomere Length in the Study on Stress, Spirituality, and Health (SSSH)
Isehunwa OO, Warner ET, Spiegelman D, Zhang Y, Palmer JR, Kanaya AM, Cole SA, Tworoger SS, Shields LO, Gu Y, Kent BV, De Vivo I, Shields AE. Depression, Religiosity, and Telomere Length in the Study on Stress, Spirituality, and Health (SSSH). International Journal Of Mental Health And Addiction 2021, 20: 1465-1484. PMID: 35747346, PMCID: PMC9211376, DOI: 10.1007/s11469-020-00455-1.Peer-Reviewed Original ResearchRelative leukocyte telomere lengthCohort-specific analysesTelomere lengthLeukocyte telomere lengthEthnic populationsQuantitative polymerase chain reactionProspective studyPolymerase chain reactionEffect modificationRace/ethnic populationsProspective associationsDepressionChain reactionAssociationEuropean ancestry populationsAncestry populationsHealthModifying influenceFurther researchPopulationResources of resilienceMixed resultsCohort
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